Journal: Journal of Cardiovascular Development and Disease

Article Title: Role of P2X7 Receptor on Hypoxia-Induced Vascular Endothelial Growth Factor Gene Expression in H9c2 Rat Cardiomyocytes

doi: 10.3390/jcdd12110438

Figure Lengend Snippet: Effect of A740003 and Echinomycin treatment on cytotoxic activity in H9c2 cells exposed to hypoxia. LDH activity in H9c2 cells exposed to 6 h of hypoxia, 5 μM A740003 + hypoxia and 1nM Echinomycin + hypoxia. Data are presented as mean ± SEM ( n ≥ 3). One-way analysis of variance (ANOVA) test was used followed by Tukey’s multiple comparisons test. **** p < 0.0001 compared to normoxia control group.

Article Snippet: A740003 and Echinomycin were procured from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Activity Assay, Control

Journal: Journal of Cardiovascular Development and Disease

Article Title: Role of P2X7 Receptor on Hypoxia-Induced Vascular Endothelial Growth Factor Gene Expression in H9c2 Rat Cardiomyocytes

doi: 10.3390/jcdd12110438

Figure Lengend Snippet: Effect of A740003 and Echinomycin treatment on VEGF level in H9c2 cells exposed to hypoxia. VEGF levels in H9c2 cardiomyocytes exposed to 6 h of hypoxia, 5 μM A740003 + hypoxia and 1nM Echinomycin + hypoxia. Data are presented as mean ± SEM ( n ≥ 3). One-way analysis of variance (ANOVA) test was used followed by Tukey’s multiple comparisons test. *** p < 0.001 compared to normoxia control group; * p < 0.05 and ** p < 0.01 compared to hypoxia group.

Article Snippet: A740003 and Echinomycin were procured from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Control

Journal: Journal of Cardiovascular Development and Disease

Article Title: Role of P2X7 Receptor on Hypoxia-Induced Vascular Endothelial Growth Factor Gene Expression in H9c2 Rat Cardiomyocytes

doi: 10.3390/jcdd12110438

Figure Lengend Snippet: Effect of A740003 and Echinomycin treatment on HIF-1α and VEGF mRNA expression level in H9c2 cells exposed to hypoxia. ( a ) HIF-1α and ( b ) VEGF mRNA expression levels in H9c2 cardiomyocytes exposed to 6 h of hypoxia, 5 μM A740003 + hypoxia and 1nM Echinomycin + hypoxia. Data are presented as mean ± SEM ( n ≥ 3). One-way analysis of variance (ANOVA) test was used followed by Tukey’s multiple comparisons test. *** p < 0.001 and **** p < 0.0001 compared to normoxia control group; *** p < 0.001 and **** p < 0.0001 compared to hypoxia group.

Article Snippet: A740003 and Echinomycin were procured from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Expressing, Control

Journal: Journal of Cardiovascular Development and Disease

Article Title: Role of P2X7 Receptor on Hypoxia-Induced Vascular Endothelial Growth Factor Gene Expression in H9c2 Rat Cardiomyocytes

doi: 10.3390/jcdd12110438

Figure Lengend Snippet: Schematic representation of the role of P2X7Rs in pro-angiogenic signaling in response to hypoxic cardiomyocytes. ( a ) Hypoxic cardiomyocytes activate P2X7R, which stimulates cardiac pro-angiogenic signaling through HIF-1α/VEGF. ( b ) A740003, a P2X7R antagonist, inhibits pro-angiogenic signaling in hypoxic cardiomyocytes by inhibiting the HIF-1α/VEGF pathway. Abbreviations: eATP, extracellular adenosine 5-triphosphate; iATP, intracellular adenosine 5-triphosphate; HIF-1α, Hypoxia-inducible factor 1 alpha; VEGF, vascular endothelial growth factor. Blue circles represent ATP, while the red circle represents the P2X7R antagonist A740003. (Created with BioRender.com ).

Article Snippet: A740003 and Echinomycin were procured from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques:

Journal: Theranostics

Article Title: Poly (I:C)-induced inflammation requires the activation of toll-like receptor 3/Ca 2+ /CaMKII/pannexin 1-dependent signaling

doi: 10.7150/thno.100687

Figure Lengend Snippet: Poly (I:C)-induced activation of pannexin1 hemichannel depends on toll-like receptor 3, Ca 2+ mobilization, and P2X 7 R activity in RAW cells. A-C. Representative records of Fura-2 fluorescence in RAW cells stimulated with Poly (I:C) (A) or assayed in combination with 30 µM TLR3i (B), or 20 µM A740003 (C). In B and C, 5 µM A23187 Ca 2+ ionophore was used for demonstrating membrane integrity. Cells were loaded with Fura-2 and recording was made in the presence of 1 mM Na 3 VO 4 (n = 3). D . RAW cells were transfected with Lck-GcamP6 and 24 h later GcamP6 fluorescence was evaluated in response to Poly (I:C) or 0.5 mM ATP (n = 3). E . Area under the curve from A-C. F . DAPI uptake rates of RAW cells assayed in response to 10 µg/mL Poly (I:C) or preincubated with 30 µM TLR3i, 20 µM A740003, 5 µM XeC or 10 µM BAPTA ** p < 0.01; *** p < 0.001.

Article Snippet: 10 Panx1, A740003, and DAPI were purchased from Tocris Bioscience (Bristol, United Kingdom).

Techniques: Activation Assay, Activity Assay, Fluorescence, Membrane, Transfection